Table 4.

Main effects of amaranth feeding, enzyme additionand interaction between amaranth feeding and enzyme addition on egg quality traits of laying hens.

Item	Shell Thickness (mm)	Shell Strength (kg/cm2)	Shape Index (%)	Egg Specific Gravity (g cm−3)	Haugh Unit	Yolk Cholesterol (mg/g yolk)	Egg Cholesterol (mg/egg yolk)
Amaranth Grain (AG), (g/kg)
0	0.306	3.323	75.04	1.083	79.66	12.59 a	218.26 a
100	0.313	3.350	75.17	1.085	80.76	11.78 ab	204.94 ab
200	0.311	3.343	75.04	1.084	80.67	11.74 ab	203.68 ab
300	0.313	3.350	74.88	1.084	80.36	11.33 b	195.02 b
400	0.303	3.355	74.82	1.083	80.17	11.30 b	194.87 b
SEM	0.005	0.025	0.919	0.001	0.666	0.329	5.730
p-value	0.85	0.99	0.99	0.95	0.78	0.04	0.04
p-value
Contrasts	0.84	0.38	0.95	0.48	0.27	0.001	0.04
Linear	0.83	0.45	0.80	0.71	0.77	0.001	0.001
Quadratic	0.85	0.76	0.91	0.73	0.26	0.34	0.38
Enzyme (±E), (g/kg)
0 (−E)	0.310	3.335	75.00	1.083	80.40	11.81	203.98
0.25 (+E)	0.311	3.354	74.99	1.085	80.25	11.70	202.73
SEM	0.003	0.016	0.581	0.001	0.421	0.208	3.624
p-value	0.83	0.42	0.99	0.33	0.80	0.72	0.81
Interactions effect (AG × E)
T1: 0 × 0	0.307	3.317	75.10	1.083	79.73	12.77	220.99
T2: 100 × 0	0.311	3.335	75.18	1.085	80.61	11.81	205.15
T3: 200 × 0	0.307	3.337	74.83	1.083	80.56	11.79	203.75
T4: 300 × 0	0.310	3.340	74.86	1.083	80.82	11.32	194.56
T5: 400 × 0	0.315	3.345	75.00	1.083	80.29	11.34	195.46
T6: 0 × 0.25	0.305	3.330	74.98	1.085	79.58	12.42	215.53
T7: 100 × 0.25	0.317	3.365	75.16	1.085	80.92	11.76	204.74
T8: 200 × 0.25	0.315	3.350	75.25	1.087	80.78	11.70	203.61
T9: 300 × 0.25	0.307	3.360	74.90	1.084	79.90	11.34	195.48
T10: 400 × 0.25	0.310	3.365	74.64	1.086	80.06	11.27	194.28
SEM	0.007	0.036	1.300	0.002	0.942	0.466	8.104
p-value	0.85	0.99	0.99	0.90	0.97	0.99	0.99

^a,^b Means within each column with different superscripts differ (p < 0.05). T₁: 0 AG-E, T₂: 100 AG-E, T₃: 200 AG-E, T₄: 300 AG-E, T₅: 400 AG-E, T₆: 0 AG + E, T₇: 100 AG + E, T₈: 200 AG + E, T₉: 300 AG + E, T₁₀: 400 AG + E.