Figure 2.

The morphological changes of Eimeria tenella oocysts after 24 and 48 h due to in vitro exposure to various treatments. Non-treated control: (A,B) Non-sporulated (thick black arrow) oocysts with regular, smooth double walls and refractile contents. (C) Sporulated tetrasporic dizoic oocysts (black star). Allicin-treated oocysts: (D) Non- oocysts were severely deformed with a ruptured wall and leaked contents (thick black arrow). (E,F) Some oocysts exhibited a shrunken appearance, lost their regular ovoid shape (black star), and contained undifferentiated contents. Alcoholic garlic extract treated oocysts: (G) The oocyst wall was corrugated (thin arrow), (H,I) The contents of the oocyst were disrupted (thick black arrow). Oocysts were treated with 5% KOH; (J–L) The oocysts’ walls were relatively undisturbed (thick black arrow), but the cyst contents were deformed. Light microscope, 40× and 100×.