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. 2022 Oct 29;11(11):2145. doi: 10.3390/antiox11112145

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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(a) The ROS levels in cells treated with various concentrations of M1 at 8 h after 4 Gy irradiation. (b) The ROS levels in M1 treated and untreated cells at 8 h after various doses of irradiation. (c) The ROS levels in M1 treated and untreated cells at various times after 4, 6, and 8 Gy irradiation. (d) Flow cytometry ROS analysis of HIEC-6 cells treated with groups of IR, IR + 0.1 mM, and IR + 0.5 mM at 24 h after 6 and 8 Gy irradiation (numbers represent ROS level). (e) Quantitative analysis of ROS level from (d). (f) Typical clone images of HIEC-6 cells belong to groups of IR, IR + 0.1 mM, and IR + 0.5 mM at 10 days after 0, 4, 6, 8, and 10 Gy irradiation. (g) Quantitative analysis of survival fractions from (f). Results are presented as the mean ± SD in triplicate and analyzed by one-way ANOVA.