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. 2022 Nov 5;11(11):2190. doi: 10.3390/antiox11112190

Table 1.

Primers used for PCR amplification.

Gene Name Accession Number Sequence Reference
SREBP-1c NM_001276707.1 Forward: 5′-GCCTGCTTGGCTCTTCTCT-3′
Reverse: 5′-GCTTGTTTGCGATGTCTCC-3′
[62]
FAS NM_017332.1 Forward: 5′-TCGACTTCAAAGGACCCAGC-3′
Reverse: 5′-ACTGCACAGAGGTGTTAGGC-3′
[63]
PPARα NM_013196.1 Forward: 5′-ATTCGGCTAAAGCTGGCGTA-3′
Reverse: 5′-TGCATTGTGTGACATCCCGA-3′
[63]
CPT1α BC072522.1 Forward: 5′-GGACATTCCTCTCTCAGGTTTC-3′
Reverse: 5′-ACCTCCTCCTTTGAACACATAC-3′
[63]
GAPDH NM_017008 Forward: 5′-TCACCACCATGGAGAAGGC-3′
Reverse: 5′-GCTAAGCAGTTGGTGGTGCA-3′
[39]

SREBP-1c, sterol regulatory element-binding protein-1c; FAS, fatty acid synthase; PPARα, peroxisome proliferator activated receptor α; CPT1α, carnitine palmitoyl transferase 1α; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.