Table 1.
Amino acid sequences, hydrophobicity (H), hydrophobic moment (µH), antimicrobial activity, and cytotoxicity of XIW peptides.
| Name | Sequence | H a | µH b | Net Charge | GM (µM) c | Cytotoxicity (%) d |
|---|---|---|---|---|---|---|
| KIW-10 | KKIIKKIWKW-NH2 | 0.495 | 0.822 | +6 | 11 ± 1 | 0 ± 0 |
| KWI-10 | KKIWKKWIKI-NH2 | 0.495 | 0.880 | +6 | 10.5 ± 1.2 | 0 ± 0 |
| WIK-10 | WIKKIWKKIK-NH2 | 0.495 | 0.902 | +6 | 6.5 ± 0.8 | 0 ± 0 |
| RIW-10 | RRIIRRIWRW-NH2 | 0.485 | 0.829 | +6 | 8.5 ± 0.8 | 0 ± 0 |
| RWI-10 | RRIWRRWIRI-NH2 | 0.485 | 0.887 | +6 | 7 ± 1.2 | 0 ± 0 |
| WIR-10 | WIRRIWRRIR-NH2 | 0.485 | 0.907 | +6 | 3 ± 0.2 | 0.8 ± 0.2 |
| KIW-14 | KKIIKKIIKKIWKW-NH2 | 0.469 | 0.782 | +8 | 2.6 ± 1.2 | 3.2 ± 0.8 |
| KWI-14 | KKIWKKWIKKIIKI-NH2 | 0.469 | 0.827 | +8 | 2.6 ± 0.8 | 1.2 ± 0.4 |
| WIK-14 | WIKKIWKKIIKKIK-NH2 | 0.469 | 0.816 | +8 | 2.1 ± 0.4 | 7.8 ± 1.2 |
| WIKE-14 | WIKKIWKKIIKEIK-NH2 | 0.494 | 0.823 | +6 | 8 ± 0.8 | 0.3 ± 0.1 |
a Mean hydrophobicity (H) and b mean relative hydrophobic moments (µH) were calculated in a HeliQuest Web server (http://heliquest.ipmc.cnrs.fr, accessed on 29 October 2022). c The geometric mean of minimum inhibitory concentration values against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, and Listeria monocytogenes. d Cytotoxicity was assayed against RAW264.7 cells at a 100 µM peptide concentration.