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. 2022 Nov 7;11(11):2199. doi: 10.3390/antiox11112199

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Modulation of Dex neuroprotection in cultured hippocampal neurons by NOX2 knockdown. (A) The purity of hippocampal neurons was evaluated by immunofluorescence detection with NeuN (red) and DAPI (blue). (B) The siRNA-mediated transfection efficiency of NOX2 was assessed using qRT-PCR. * p < 0.05 versus the Scrambled group. (C) Intracellular ROS production was detected using the DCFH-DA probe. (D) The intensity of ROS fluorescence was analyzed using ImageJ. (E,F) Apoptosis was detected and analyzed using flow cytometry by staining cells with FITC-Annexin V/PI. Results are expressed as mean ± SD (n = 4 in each group). Each data point in the bar graph is also presented. * p < 0.05 versus the Hypoxia + Scrambled group.