Table 1.
* Physicochemical characteristics of AZI PLGA NPs, each value is an average of three replicates.
| AZI Initial Weight | ||||
|---|---|---|---|---|
| (mg)/Formula Name | Aqueous Phase | ** %DL ± SD | ** PS (D nm) ± SD (PDI) | ** ZP (mV) ± SD |
| Blank | Water | ------ | 188.5 ± 51.92 (0.193) | −24.83 ± 6.85 |
| 10/F1 | Water | 3.88 ± 0.07 | 225.5 ± 128.7 (0.276) | −27.9 ± 6.61 |
| 15/F2 | Water | 3.43 ± 0.11 | 184.5 ± 115 (0.253) | −30.1 ± 6.61 |
| 20/F3 | Water | 3.47 ± 0.24 | 101 ± 25.01 (0.181) | −34.8 ± 6.61 |
| 50/F4 | Water | 3.52 ± 0.23 | 593.7 ± 244.4 (0.651) | −29.5 ± 6.61 |
| 10/F5 | Water/Tween 80 (0.1%) | 5.74 ± 0.28 | 134 ± 57.53 (0.198) | −32.5.2 ± 4.87 |
| 15/F6 | Water/Tween 80 (0.1%) | 3.43 ± 0.14 | 114.3 ± 52.76 (0.266) | −35.7 ± 6.61 |
| 20/F7 | Water/Tween 80 (0.1%) | 4 ± 0.16 | 112.3 ± 42.08 (0.279) | −34.2 ± 6.61 |
| 50/F8 | Water/Tween 80 (0.1%) | 2.88 ± 0.34 | 154.9 ± 78.03 (0.269) | −30.2 ± 6.61 |
| 10/F9 | Phosphate buffer (10 mM PH 6) | 3.52 ± 0.23 | 118.4 ± 56.27 (0.204) | −32.7 ± 6.45 |
| 15/F10 | Phosphate buffer (10 mM PH 6) | 4.78 ± 0.13 | 107.5 ± 57.97 (0.307) | −33.3 ± 6.62 |
| 20/F11 | Phosphate buffer (10 mM PH 6) | 3.78 ± 0.28 | 105.8 ± 42.6 (0.367) | −30.9 ± 5.34 |
| 50/F12 | Phosphate buffer (10 mM, PH 6) | 3.35 ± 0.16 | 521.6 ± 158.4 (0.598) | −31.6 ± 6.3 |
| 10/F13 | Phosphate buffer (10 mM PH7.4) | 4.59 ± 0.27 | 111.4 ± 42.95 (0.301) | −41.8 ± 5.79 |
| 15/F14 | Phosphate buffer (10 mM PH7.4) | 4.35 ± 0.12 | 142.2 ± 58.73 (0.224) | −35.2.1 ± 5.87 |
| 20/F15 | Phosphate buffer (10 mM PH7.4) | 4.55 ± 0.26 | 154.3 ± 98.39 (0.316) | −34.7 ± 8.08 |
| 50/F16 | Phosphate buffer (10 mM PH7.4) | 4.32 ± 0.45 | 416 ± 50 (0.595) | −43.6 ± 6.65 |
| 10/F17 | Phosphate buffer (10 mM PH 8) | 3.19 ± 0.18 | 142.6 ± 67.46 (0.272) | −52.5 ± 7.8 |
| 15/F18 | Phosphate buffer (10 mM PH 8) | 3.49 ± 0.25 | 348.8 ± 104.1 (0.584) | −41.1 ± 4.99 |
| 20/F19 | Phosphate buffer (10 mM PH 8) | 4 ± 0.67 | 534.3 ± 246.7 (0.626) | −40.9 ± 5.06 |
| 50/F20 | Phosphate buffer (10 mM PH 8) | 3.13 ± 0.07 | 673 ± 151 (0.437) | −56.1 ± 8.06 |
* Data were analyzed by two-way ANOVA; there was non-significant (p > 0.05) differences between samples concerning all measured physicochemical parameters. ** Physicochemical parameters measured were; percentage of drug loading (DL%), particle size (PS), Diameter in nm (D nm), polydispersity index (PDI), zeta potential (ZP), standard deviation (SD).