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. 2022 Nov 17;11(11):2279. doi: 10.3390/antiox11112279

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Characteristics of UCMSC-EVs. (A) Size distributions of UCMSC-EVs based on NTA measurements. The mean diameters were 103.3 ± 1.0 nm. (B) EVs were negatively stained and viewed by transmission electron microscopy at a magnification of 60,000. The scale bar represents 200 nm. (C) Representative immunoblot showing the expression of the EVs’ markers CD9, CD63, CD81, Tsg101 and Alix, whereas HSP70, calnexin, annexin V and β-actin acted as negative control.

Characteristics of UCMSC-EVs. (A) Size distributions of UCMSC-EVs based on NTA measurements. The mean diameters were 103.3 ± 1.0 nm. (B) EVs were negatively stained and viewed by transmission electron microscopy at a magnification of 60,000. The scale bar represents 200 nm. (C) Representative immunoblot showing the expression of the EVs’ markers CD9, CD63, CD81, Tsg101 and Alix, whereas HSP70, calnexin, annexin V and β-actin acted as negative control.