FIG. 3.

Conditioned media from S. enteritidis CD5 (●), S. typhimurium SL1344 (▿), Y. enterocolitica JB580 (■), P. aeruginosa PAO1 (◊), and enteropathogenic E. coli E2348/69 (▴) induce TNFα production in LPS-tolerized PBMC. Following overnight exposure to LPS (1 μg/ml), PBMC were stimulated with conditioned medium from each strain at the dilutions indicated or with LPS (1 μg/ml). The amount of TNFα in each sample (cell associated and released) was measured by ELISA after a 4-h incubation. The data are representative of three similar experiments. The horizontal line represents the amount of TNFα produced in response to LPS (1 μg/ml) in the experiment shown.