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. 2022 Nov 17;12(22):3186. doi: 10.3390/ani12223186

Table 1.

Primers used in the qRT-PCR analysis performed to validate the expression profiles of the studied genes.

Gene Oligo Forward Sequence (5′-3′) Oligo Reverse Sequence (5′-3′)
Erα CACCCTGGAAAGCTGTTGTT TTCGGAATCGAGTAGCAGTG
Erβ ATCCCGGCAAGCTAATCTTT CAGCTCTCGAAACCTTGAAGT
GnRH GTCTTGCTGGCCTCTCCTC GTGGTCTCCTGCCAGTGTTC
P450 aromatase TGAACACCCTCAGTGTGGAA TCAGVTTTGGCATGTCTTCA
PRL AAGGCCATGGAGATTGAGG GGAGGCCTGACCAAGTAGAA
PRL-R ATGGAGGTCTCCCCACTAAT AACAGGAATTGGGTCCTCCT
WNT4 CTGCAACAAGACCTCCAAGG AGCAGCACCGTGGAATTG
SOX9 GGGCAAGCTTTGGAGGTTAC TGGGCTGGTACTTATAGTCTGGA
DMRT1 GCAGGGGATCCTACCAAAGT AGAAGGCAGCAAGCTCAAGA
ALDOC TACCATGGTGTTGTGCAAGC CTTCACGCTGCATTTTCTCA
FREM1 GGAATGTCAACCAAGATGTGG CAGGGGAGATCAGAACCACT
BBIP1 CGTGAGCTGTAGCTTTGCAG CTGCCTTACCCACAGCACTT
CA5A TTGCAAAGTTATGGGGAGGA TCAAGCAGGGTTTATTTCTCATC
NADH5 GCTACAGGTAAATCCGCTCAA AGTAGGGCAGAAACGGGAGT
L1 non-LTR retrotransposons ATCATCGTGGGCCTCTTTGC AGCAGCACCGTGGAATTG
PKM AGAGCTGCTTGTACGCCTGT CCAGATTTCCAAAGGACAGTG