Table 4.
Summary of the differentially expressed genes in E. macularius embryo at stage 30, listed according to the two different methodologies used. MPT stands for genes more expressed at a male-producing temperature and FPT stands for genes more expressed at a female-producing temperature.
| Gene | Identification | Embryo Gonads Stage 30 |
|---|---|---|
| Erα | qRT-PCR | No differential expression |
| Erβ | qRT-PCR | No differential expression |
| GnRH | qRT-PCR | No differential expression |
| P450 aromatase | qRT-PCR | No differential expression |
| PRL | qRT-PCR | Not detected |
| PRL-R | qRT-PCR | FPT |
| WNT4 | qRT-PCR | MPT |
| SOX9 | qRT-PCR | MPT |
| DMRT1 | qRT-PCR | MPT |
| ALDOC | DDRT-PCR | MPT |
| FREM1 | DDRT-PCR | MPT |
| BBIP1 | DDRT-PCR | MPT |
| CA5A | DDRT-PCR | FPT |
| NADH5 | DDRT-PCR | No differential expression |
| L1 non-LTR retrotransposons | DDRT-PCR | FPT |
| PKM | DDRT-PCR | MPT |