Figure 4.
Liposomal STS maintains pro-angiogenic signals of H2S under conditions mimicking hypoxia established by CoCl2. EA.hy926 were exposed to CoCl2 (400 μM) in combination with either non-formulated or liposome formulated STS for 24 h. (A) Expression of VEGF mRNA measured by qPCR. (B) VEGF release measured by ELISA in cell culture media. (C) Expression of Glut-1 mRNA measured by qPCR. (D) Capillary-like formation measured as total branching length was investigated in HUVEC exposed to CoCl2 (400 μM) in combination with either non-formulated or liposome formulated STS and calculated using Image J angiogenesis tool. (E) Cell migration measured as percentage of wound closure relative to 0 h. (F) Images representative of tube formation assays captured at 4×. Data represents mean ± SD (n = 3). Statistical differences: * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.