Figure 6.
(A1,A2) Testis of the control rat showing weak TNF-α immuno-stain in the cytoplasm of few spermatogenic cells. (B1,B2) Testis of corn oil-treated rat showing weak expression for TNF-α. (C1,C2) Testis of QTN-treated rat showing weak immuno-labeling of some spermatogenic cells. (D1,D2) Testis of Al2O3NPs-exposed group showing numerous positive spermatogenic cells and Leydig cells represented by brown color. (E1,E2) Testis of the PbAc-exposed group showing a strong positive reaction in the cytoplasm of spermatogenic cells and Leydig cells. (F1,F2) Testis of Al2O3NPs and PbAc co-exposed group showing strong positive TNF-α immuno-stain (G1,G2) Testis of Al2O3NPs and PbAc co-exposed and QTN-treated group showing weak TNF-α positive cells (TNF-α immuno-stained, ×100 for (A1–G1) and ×400 (A2–G2)). Yellow arrows denoted TNF-α-positive immune stained cells.