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. 2022 Nov 8;149(21):dev200920. doi: 10.1242/dev.200920

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© 2022. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 5. — meg-1/2 are required for the turnover of a subset of POS-1 targets. (A) RNA-seq from two independent experiments comparing meg-1 meg-2 (RNAi) and wild-type embryos identified 230 downregulated and 550 upregulated genes (±1.5 fold change). Purple dots correspond to genes significantly down/upregulated in meg-1 meg-2 embryos that also exhibited longer poly-A tails in pos-1(RNAi) embryos (Elewa et al., 2015). (B) A total of 223 genes upregulated in meg-1 meg-2 embryos overlap with genes with poly-A tails extended in pos-1(RNAi) embryos. P=0.0002 (Fisher's exact test; Materials and Methods). (C,E) Photomicrographs of cdc-25.3 and neg-1 smFISH in embryos expressing the P granule marker MEG-3::GFP. Inset shows P₄. cdc-25.3 and neg-1 are turned over less efficiently in meg-1 meg-2 P₄ blastomeres. Dashed white line indicates embryo boundary. Dashed square indicates P₄. (D,F) Intensity of cdc-25.3 and neg-1 in P₄ normalized to wild type. In situs for cdc-25.3 and neg-1 were done in the same embryos in two independent experiments in which mutant and control animals were processed in parallel. Wild type n=29; meg-1/2 n=38. Data are mean±s.d. Unpaired two-tailed t-test was used to make comparisons between genotypes. Scale bars: 1 µm.