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. 2022 Nov 10;11(11):1644. doi: 10.3390/biology11111644

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Agar well diffusion assay zone sizes for the inhibition of (A) Aeromonas hydrophila (10⁶ CFU mL⁻¹), (B) Streptococcus agalactiae (10⁶ CFU mL⁻¹), (C) Vibrio harveyi (10⁶ CFU mL⁻¹), and (D) Vibrio parahaemolyticus (10⁶ CFU mL⁻¹) using single- and multi-strain probiotics. L11 = Bacillus amyloliquefaciens; LAB3 = Enterococcus hirae; SPS11 = Lysinibacillus fusiformis; NAS32 = Lysinibacillus sphaericus; A1 = Lysinibacillus fusiformis; MSP1 = L11 + LAB3 + SPS11; MSP2 = SPS11 + NAS32 + A1; Chloramphenicol = Positive control. The values shown are the size of the inhibition zone ± SE (n = 3). Different alphabetical letters (^{a, b, c, ab, bc}) indicate a statistically significant difference (p < 0.05).

Agar well diffusion assay zone sizes for the inhibition of (A) Aeromonas hydrophila (10⁶ CFU mL⁻¹), (B) Streptococcus agalactiae (10⁶ CFU mL⁻¹), (C) Vibrio harveyi (10⁶ CFU mL⁻¹), and (D) Vibrio parahaemolyticus (10⁶ CFU mL⁻¹) using single- and multi-strain probiotics. L11 = Bacillus amyloliquefaciens; LAB3 = Enterococcus hirae; SPS11 = Lysinibacillus fusiformis; NAS32 = Lysinibacillus sphaericus; A1 = Lysinibacillus fusiformis; MSP1 = L11 + LAB3 + SPS11; MSP2 = SPS11 + NAS32 + A1; Chloramphenicol = Positive control. The values shown are the size of the inhibition zone ± SE (n = 3). Different alphabetical letters (^{a, b, c, ab, bc}) indicate a statistically significant difference (p < 0.05).