Table 4.
MSI application in the research of monocultured microbial NPs or metabolites.
| Species | Ionization Method | Compound | Research Purpose | Reference |
|---|---|---|---|---|
| Actinomyces sp. CNS 575 | MALDI | Etamycin | Identification | [78] |
| Beauveria bassiana ATCC 7159 | MALDI | Beauvericin, bassianalide | Identification | [78] |
| Nostoc sp. | MALDI | Pheophytin A | Identification | [78] |
| Streptomyces coelicolor A3 | MALDI | SapB, CDA | Identification | [78] |
| Fusarium sp. CNL-292 | MALDI | Sansalvamide | Identification | [78] |
| Streptoverticillium griseoverticillatum ATCC 31499 | MALDI | Cinnamycin | Identification | [78] |
| Bacillus subtilis 3610 | MALDI | Surfactin, plipastatin | Identification | [78] |
| Lysobacter enzymogenes C3 | MALDI | Maltophilin, dihydromaltophilin | Identification | [78] |
| Staphylococcus aureus | MALDI | δ-toxin | Identification | [78] |
| Candida albicans | SIMS | Chlorhexidine digluconate (CHG) | Distribution of the CHG within the biofilms | [82] |
| Streptomyces coelicolor | SIMS | Undecylprodigiosin, butylcyclohexylprodiginin, actinorhodins |
Antibiotic distribution on the cell surface | [83] |
| Bacillus subtilis | SIMS | Surfactins | Localization and quantification | [84] |
| Streptomyces griseus IFO 13350 | MALDI | Lantipeptide AmfS | Discovery of new natural products | [85] |
| Streptomyces hygroscopicus ATCC 53653 | MALDI | StendomycinⅠ-Ⅵ | Discovery of new natural products | [85] |
| Streptomyces sviceus ATCC 20983 | MALDI | Lasso peptide SSV-2083 | Discovery of new natural products | [85] |
| Bacillus amyloliquefaciens S499 | SIMS | Surfactins, iturins and fengycins | Characterization of the interaction between cyclic lipopeptides with the plant | [86] |
| Staphylococcus aureus USA300 TCH1516 | MALDI | Phenol-soluble modulins | Study the metabolism of community-associated methicillin-resistant Staphylococcus aureus | [87] |
| Mycena metata | MALDI | 6-Hydroxymetatacarboline D | Discovery of new natural products | [88] |
| Pseudomonas aeruginosa | MALDI | P. aeruginosa derived metabolites | Microbial metabolite manner under the treatment of azithromycin | [89] |
| Herpetosiphon sp. B060 | MALDI | Siphonazole | Biosynthetic pathway | [90] |
| Microcystis aeruginosa PCC 7820 | MALDI | Aeruginosin 602, microcystin-LR | Distribution | [91] |
| Nodularia. harveyana PCC 7804 | MALDI | Anachelin | Distribution | [91] |
| Anabaena Cylindrica PCC 7122 | MALDI | Nodularin-R, nodularin-[Har] | Distribution | [91] |
| Streptomyces sp. Mg1 | MALDI | Linearmycins | Localization | [92] |
| Pseudomonas aeruginosa | SIMS | Alkyl-quinolone | Quantitation | [93] |
| Penicillium digitatum | DESI | Indole alkaloids | Characterization and identification | [94] |
| Streptomyces sp. Caat 1-54 | DESI | Lienomycin, lysolipin I | Characterization and identification | [95] |
Note: DESI: desorption electrospray ionization; MALDI: matrix-assisted laser desorption/ionization; SIMS: secondary ion mass spectrometry.