Table 5.
MSI application in the research of microbial binary interaction.
| Species | Ionization Method | Compound | Research Purpose | Reference |
|---|---|---|---|---|
| Bacillus subtilis vs. Streptomyces coelicolor | MALDI | Surfactin, plipastatin, Prodiginines, CDA, SapB |
Characterization of bacterial metabolic exchange | [111] |
| Bacillus subtilis vs. Streptomyces coelicolor | DESI | Actinorhodin, surfactin, plipastatin |
Bacterial interaction | [57] |
| Bacillus subtilis | MALDI | Sporulation-delaying protein (SDP), sporulation killing factor (SKF) | Intraspecies interaction | [112] |
| Streptomyces roseosporus vs. Streptomyces epidermidis | MALDI | Arylomycins | Discovery of bioactive NPs | [74] |
| Bacillus subtilis vs. Staphylococcus aureus | MALDI | Surfactin, plipastatin, PSMλ, PSMα3 | Microbial competition | [113] |
| Streptomyces sp. Mg1 vs. Bacillus subtilis | MALDI | Polyglutamate, surfactin, plipastatin, chalcomycin A | Microbial competition | [114] |
| Streptomyces sp. Mg1 vs. Bacillus subtilis | MALDI | Surfactin, hydrolyzed surfactin | Interspecies competition | [115] |
| Streptomyces coelicolor vs. other five species actinomycetes | MALDI and nano-DESI | Acyl-desferrioxamine siderophores | Interspecies interaction | [116] |
| Endophytic bacteria of Cannabis sativa L vs. Chromobacterium Violaceum | MALDI | N-acylated L-homoserine lactones (AHLs) | Quorum sensing | [117] |
| Pseudomonas aeruginosa vs. Staphylococcus aureus | MALDI | Pyocyanin, rhamnolipid, 4-hydroxy-2-alkylquinoline (HAQ) derivatives | Metabolic profile and interspecies interaction | [118] |
| Paraconiothyrium variabile vs. Bacillus subtilis | MALDI | Surfactin | Investigation of antagonism | [119] |
| Paenibacillus dendritiformis vs. Bacillus subtilis NCIB3610 | MALDI | Surfactin and its degradations | Specific interaction of attractant | [120] |
| Myxococcus xanthus DK1622 vs. Escherichia coli | DESI | Myxovirescin A, DKxanthene-560 |
Investigation of predation process | [73] |
|
Janthinobacterium agaricidamnosum vs. Agaricus bisporus |
MALDI | Jagaricin A | Investigation of the virulence factors of soft rot bacteria | [121] |
| Pseudomonas aeruginosa vs. Aspergillus fumigatus | MALDI | Fungal siderophores, phenazine metabolites |
Interkingdom metabolic transformation | [122] |
| Paenibacillus polymyxa (Pp56) vs. Fusarium oxysporum | MALDI | Fusaricidins A, B, C, lipopeptides |
Searching for microbial biocontrol agents | [123] |
| Fusarium solani vs. Achromobacter xylosoxidans | MALDI | Hexacyclopeptides | Discovering metabolites produced by endophytes | [124] |
| Ralstonia solanacearum vs. Aspergillus flavus | MALDI | Ralsolamycin | Interkingdom interaction | [125] |
| Burkholderia seminalis vs. Moniliophthora perniciosa, Phytophthora capsisi, Phytophthora palmivora and Phytophthora citrophtora | DESI | Phospholipids, rhamnolipid |
Searching for cacao pathogens’ biocontrol agents | [126] |
| Streptomyces sp. (CB0028) vs. Escovopsis sp. (CBAcro424) | MALDI | Siderophores | Searching for natural products in microbial interaction | [127] |
| Ralstonia solanacearum vs. Fusarium fujikuroi and Botrytis cinerea | MALDI | Ralsolamycin, bikaverin | Small molecular induced microbial interaction | [128] |
| Burkholderia cenocepacia 869T2 vs. Phellinus noxius | MALDI | Pyochelin and its esterification product | Dynamic changes of metabolites in microbial interactions | [109] |
| Staphylococcus aureus vs. Pseudomonas aeruginosa | MALDI | Pyochelin, pyochelin methylester | Bacterial competition in vivo | [110] |
| Trichoderma harzianum vs. Moniliophthora roreri | DESI | T39 butenolide, harzianolide, sorbicillinol | Investigation of antagonistic interaction of fungi | [129] |
| Phellinus noxius vs. Aspergillus sp. 3Y and 3G | SALDI | Sterigmatocystin, fellutamides |
Developing microbial MSI on nanostructured silicon | [54] |
|
Penicillium polonicum ACCC31573 vs. Fusarium oxysporum f. sp. lycopersici 4287 |
MALDI | Fructigenine A and B | Screening for antifungal antibiotics | [130] |
| Penicillium digitatum vs. Penicillium citrinum | DESI | Tryptoquialanines, 15-dimethyl-2-epi-fumiquinazoline A, deoxytryptoquialanone, citrinadin A, deoxycitrinadin A, chrysogenamide A, tetrapeptide |
Screening for new antifungal compounds | [131] |
| Purpureocillium lilacinum vs. Botrytis cinerea | MALDI | Leucinostatin Z | Searching for new natural products | [132] |
Note: DESI: desorption electrospray ionization; MALDI: matrix-assisted laser desorption/ionization; SALDI: surface-assisted laser desorption/ionization.