Figure 1.

The effect of sodium lactate on rotenone-induced cell toxicity. Rotenone was added to the rat brain cell culture 36 h before measurements; 12 h after the addition of rotenone, sodium lactate was added. (A–C) Representative images of cells in primary cortical neuroglial culture stained with Hoechst 33342 (blue) and propidium iodide (red). (A) Control conditions (B) After 36 h of 1 mg/mL rotenone exposure (C) Rotenone in combination with 10 mM sodium lactate. Scale bar: 100 μm. (D) Percentage of nonviable cells, n = 3 experiments, ** p < 0.01.