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. 2022 Nov 4;12(11):1638. doi: 10.3390/biom12111638

Figure 5.

Figure 5

Genome editing using Gelatin-Ph nanofibers with Lipofectamine/pDNA complexes. (a) Schematic representation of genome editing reporter assays for gene knock-in and knock-out. EF1α promoter drives GFP or mutated GFP. Red pentagon, Cas9/gRNA target sequence. Black line within the red pentagon, Cas9 cleavage site. tGFP, HDR-donor carrying truncated GFP. HDR, Homology-directed repair. NHEJ, Non-homologous end joining. (b) Genome editing rates of gene knock-in and knock-out. Data represent mean ± SD (n = 5). (c,d) Representative flow cytometry images showing the gene knock-in (c) and knock-out (d) rates.