Figure 2.

General observations on the CHX-induced mouse model. (a) The body weight of CHX-treated, treated control, and untreated control mice throughout the 1-week and 3-week experiment. Body weight of mice in 1-week experiment: no changes were seen in the group of untreated control mice (from 26.4 ± 1.3 g to 26.6 ± 1.2 g, p = 0.551 (n = 3)) and control mice (26.3 ± 2.4 g to 26.5 ± 1.5 g, p = 0.659 (n = 4)), while there was a decrease in the group of CHX-treated mice (27.7 ± 2.5 g to 26.5 ± 2.3 g, p = 0.024 (n = 5)). Similar results were observed when comparing the weight of mice in 3-week experiment; in control mice, weight did not change over time (from 26.1 ± 2.1 g to 26.2 ± 2.1 g, p = 0.921 (n = 4)), while there was a progressive weight loss over time in the group of CHX-treated mice (26.6 ± 1.5 g to 24.9 ± 2.159 g, p = 0.017 (n = 5)). (b) Macroscopic changes in the liver and the abdominal wall in the control, 1-week, and 3-week CHX-treated mice. Macroscopic changes in organs and tissues of the abdominal cavity were already seen at the 1-week time point and progressed severely to the 3-week time point. The arrow shows the attachment of mesentery to the abdominal wall present in 1/5 CHX-treated mice. Created with BioRender.com. (c) The relative weights of the liver, the spleen, and the kidney, normalized to the total animal weight, did not differ between the untreated controls, treated controls, and CHX-treated mice. Legend: CHX: chlorhexidine gluconate, ctrl: control, untr. ctrl: untreated control.