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. 2022 Nov 1;10(11):2773. doi: 10.3390/biomedicines10112773

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Morphology, proliferation, and surface markers of HUCMSCs cultured with exosome-depleted (Exo(-)FBS) and conventional fetal bovine serum (FBS). (A) Morphology of the Exo(-)FBS- and FBS-cultured HUCMSCs showed fibroblastic and spindle-shaped on days 1 and 4. Scale bar = 100 μm. (B) Cell proliferation curve of Exo(-)FBS- and FBS-cultured HUCMSCs. ** p < 0.01. Representative results from 3 experiments. (C) Flow cytometry of Exo(-)FBS- and FBS-cultured HUCMSCs. They are negative for CD34, CD45, and HLA-DR and positive for CD44, CD73, CD90, CD105, and HLA-ABC and Exo-: exosome-depleted FBS. HUCMSCs were derived from the same donor. (D) Dot plot FSC/SSC (forward scatter (size)/side scatter (granularity)) of CD44 expression in HUCMSCs culture with FBS and Exo(-)FBS. (E) HUCMSCs cultured with FBS or Exo(-)FBS were stained with FITC-Annexin V/PI and analyzed by fluorescence microscopy. Scale bar = 100 μm. DAPI: nuclear staining, PI: propidium iodide.