Table 5.
EV-miRNAs involved in homeostasis of OA-affected tissues and cells.
| TISSUE/CELLS | % WEIGHT | ROLE |
|---|---|---|
| CARTILAGE | ||
| Protective | ||
| hsa-miR-24-3p | 13.17 | Prevents ECM degradation, increases chondrocyte viability |
| hsa-miR-193b-3p | 8.39 | Reduces cartilage degradation |
| hsa-miR-222-3p | 7.59 | Reduces cartilage degradation |
| hsa-miR-320a-3p | 3.47 | Increases chondrocyte viability |
| hsa-miR-125b-5p | 1.23 | Prevents aggrecan loss |
| hsa-miR-17-5p | 0.82 | Induces autophagy |
| hsa-miR-221-3p | 0.57 | Prevents ECM degradation |
| hsa-miR-92a-3p | 0.48 | Increases collagen deposition |
| hsa-miR-199a-3p | 0.23 | Anti-catabolic |
| Total | 35.94 | |
| Destructive | ||
| hsa-miR-21-5p | 0.88 | Negatively regulates chondrogenesis |
| hsa-miR-16-5p | 0.82 | Cartilage degradation |
| hsa-miR-30b-5p | 0.44 | Pro-apoptotic, ECM degradation |
| hsa-miR-138-5p | 0.34 | Cartilage degradation |
| hsa-miR-483-5 | 0.26 | Chondrocyte hypertrophy, ECM degradation and cartilage angiogenesis |
| hsa-miR-146a-5p | 0.19 | Activator in early OA |
| hsa-miR-34a-5p | 0.17 | Apoptosis |
| Total | 3.10 | |
| Dual | ||
| hsa-miR-145-5p | 1.25 | Regulates chondrocyte proliferation and fibrosis |
| SYNOVIUM | ||
| Protective | ||
| hsa-miR-29a-3p | 0.64 | Anti-fibrotic effects |
| Destructive | ||
| hsa-miR-34a-5p | 0.17 | Enhances synovial inflammation |
| Dual | ||
| hsa-miR-146a-5p | 0.19 | Enhances/Suppresses synovial inflammation |
| MACROPHAGE | ||
| M1 | ||
| hsa-miR-145-5p | 1.25 | Pro-M1 |
| hsa-miR-125b-5p | 1.23 | Pro-M1 |
| Total | 2.48 | |
| M2 | ||
| hsa-miR-24-3p | 13.17 | Pro M2, blocks M1 |
| hsa-miR-222-3p | 7.59 | Pro M2 |
| hsa-let-7b-5p | 0.71 | Pro M2 |
| hsa-miR-146a-5p | 0.19 | Pro M2, blocks M1 |
| hsa-miR-34a-5p | 0.17 | Pro M2 |
| Total | 21.83 | |
| T CELL | ||
| Pro-Activation | ||
| hsa-miR-19b-3p | 1.35 | Reduces PTEN repressor |
| hsa-miR-214-3p | 1.32 | Reduces PTEN repressor |
| hsa-miR-21-5p | 0.88 | Reduces PTEN repressor |
| hsa-miR-106a-5p | 0.83 | Represses IL10 |
| hsa-miR-17-5p | 0.82 | Reduces PTEN repressor and promotes IFNγ |
| hsa-let-7b-5p | 0.71 | Represses IL10 |
| hsa-miR-221-3p | 0.57 | Downregulates PIK3R1 |
| hsa-miR-132-3p | 0.40 | Downregulates PIK3R1 |
| Total | 6.88 | |
| Anti-activation | ||
| hsa-miR-24-3p | 13.17 | Represses IFNγ in activated CD4+ and CD8+ |
| hsa-miR-125b-5p | 1.23 | Maintains T cell naïve state |
| hsa-miR-342-3p | 0.90 | Downregulated upon activation |
| hsa-miR-146a-5p | 0.19 | Represses activation markers |
| Total | 15.48 | |
| Dual | ||
| hsa-miR-31-5p | 0.21 | Upregulates IL2, downregulated with activation |