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Wdr5 is upregulated in Ang-II-stimulated NRCFs. (A) Col1a1, Col3a1, and α-SMA mRNA levels were upregulated after Ang II stimulation. (B) Col1a1, α-SMA, Wdr5, and H3K4me3 protein levels were upregulated in respond to Ang II. (C) is the quantitative statistic of (B) graph. (D) Immunofluorescence data of H3K4me3 in NRCFs (Scale bar 100 μm). (E) is the quantitative statistic of (D) graph. Data are presented as mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 vs. saline.
