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. 2022 Oct 27;12(11):1574. doi: 10.3390/biom12111574

Figure 3.

Figure 3

Pharmacologic inhibition of Wdr5 suppressed the FMT process. (A) Application of MM-401 and OICR-9429 repressed the Ang-II-induced col1a1, col3a1, and α-SMA. (B) Protein levels of col1a1 and α-SMA were downregulated after the application of MM-401 and OICR-9429. (C) is the quantitative analysis of graph (B). (D) Immunofluorescence analysis of α-SMA demonstrated inhibited α-SMA by OICR-9429 (green, α-SMA; blue, DAPI, Scale bar 100 μm). (E) is the quantitative statistics of graph (D) (n = 5 in each group, >3 fields in each sample). (F) Representative images of transwell migration assay and the quantification of migrated NRCFs (n = 5 in each group, >3 fields in each sample). (G) Wound repair assays were performed to assess the migratory capacities of cells that have been treated with DMSO or OICR-9429 (n = 5 in each group, >3 fields in each sample). Scale bar 100 μm. Data are presented as mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 vs. saline, # p < 0.05, ## p < 0.01 vs. Ang II group.