Figure 5.

Wdr5 inhibition attenuated fibroblasts proliferation and induced cell senescence. (A) OICR-9429 application for 48 h inhibited the Edu incorporation in fibroblasts compared with a control group (n = 4 in each group, >3 fields for each sample) (B) Sh-Wdr5 infection for 48 h inhibited the Edu incorporation in fibroblasts compared with Sh-NC (n = 4 in each group, >3 fields for each sample). (C,D) CCK8 assay demonstrated that cell viabilities were inhibited after pharmacologic and genetical inhibition of Wdr5 (n = 5 in each group). (E) SA-β Gal staining of OICR9429 or Sh-Wdr5 treated NRCFs. (F) mRNA levels of genes related to senescence-associated secretory phenotype. Scale bar 100 μm. Data are presented as mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 between the 2 indicated groups.