Pharmacologic inhibition of Wdr5 by OICR-9429 induced selective fibroblast senescence and attenuated the Ang-II-induced fibrosis. (A) Representative fluorescent images of P21, vimentin (a marker of fibroblasts), and DAPI in heart after treatment of OICR9429 and Ang II (Scale bar 50 μm). (B) is the quantitative analysis of graph (A). (C,D) Masson’s trichrome staining showed attenuated fibrosis by treatment of OICR-9429 in the models of Ang II or ISO in different areas. (E,F) OICR9429 did not affect the ventricular systolic function by measuring EF and FS. Data are presented as mean ± SEM. Scale bar 100 μm. ** p < 0.01, #### p < 0.0001 vs. Ang-II group.