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. 2022 Nov 5;9(11):655. doi: 10.3390/bioengineering9110655

Figure 3.

Figure 3

Self-organization of BM-MSCs, PBMCs, and SH-SY5Y cells in co-culture on untreated plastic and Matrigel. (A–C,G–I) Phase contrast light microscopy. (D–F,J–L) Fluorescence microscopy. (A–F) Double co-culture contains SH-SY5Y cells (FITC, green) and BM-MSCs (Alexa Fluor 647, red). (G–L) Triple co-culture contains SH-SY5Y cells (PE, yellow), BM-MSCs (Alexa Fluor 647, red), and PBMCs (FITC, green). (D–F,J–L) Fluorescence microscopy: alignment of the fluorescence spectra of Alexa Fluor 647 (BM-MSCs, red), PE (SH-SY5Y cells, yellow), and FITC (PBMCs, green). Scale: 200 µm.