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. 2022 Oct 23;12(11):1546. doi: 10.3390/biom12111546

Figure 5.

Figure 5

TAX attenuates ISO-induced myocardial NF-κB p65 upregulation. Images of microscopic field of IHC labeled cardiac sections from (A) control and (B) TAX-treated mice showing mild expression of myocardial NF-κB p65 (arrows designate positive immunostaining within the interstitial cells); (C) ISO-injected mice showing a marked increase in both cytoplasmic and nuclear levels of NF-κB p65 antibody within myocytes (arrows designate marked expression within both degenerated fibers and inflammatory cells); (D) ISO-administrated mice pre-treated with 25 mg TAX demonstrating a decreased level of the nuclear NF-κB p65 within the myocytes (arrows); and (E) ISO-administrated mice pre-treated with 50 mg TAX demonstrating a noticeably decreased expression of the nuclear NF-κB p65 within the myocardial fibers (arrows) (IHC, X200, Scale bar = 50 µm). (F) Analysis of the relative intensities of the images of IHC labeled myocardial NF-κB p65 showing a significant increase in ISO-injected mice and a significant reduction in mice pre-treated with both doses of TAX. Data are expressed as mean ± SEM, (n = 6). Here, a indicates significant (p < 0.05) vs. control, while b indicates significant (p < 0.05) vs. ISO.