Figure 6.

Effects of peptide 6027 on murine T cells (A) and B cells (B) with respect to viability and proliferation in vitro; 96-well plates were seeded with splenocytes obtained from murine spleen (2 × 10⁶ cells/mL, 100 µL/well), and were exposed to ConA (5 µg/mL) or LPS (10 µg/mL). Peptide 6027 was added at 0.1, 0.5, 1 and 2 µM, respectively, and cultivation was carried out for 48 h at 37 °C/5% CO₂. Viability was assessed by resazurin reduction and is given as relative fluorescence units (RFU); the proliferation of cells was measured by BrdU ELISA and is given as absorption at 450 nm (A₄₅₀).