Figure A3.

Gene expressions of polarized human monocyte-derived macrophages. Negatively selected monocytes (n, sample = 3; n, biological = 2; n, technical = 3) were differentiated to macrophages using M-CSF or GM-CSF (highlighted in grey). After 6 days, MΦ were stimulated with IFN-γ/LPS (M1), IL-4 (M2), or with no additive (M0). Cells were harvested after a polarization time of 48 h. The relative gene expressions of (A) IL1B, (B) IL8, (C) HSPG2 and (D) SDC2 were determined using qRT-PCR. The data were analyzed using a one-way ANOVA, followed by Tukey’s multiple comparison tests, and expressed as mean ± SEM. ns (not significant), p < 0.05 (*), p < 0.01 (**), p < 0.001 (***), p < 0.0001 (****).