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HSP90 inhibition and intracellular PD-1 and CTLA-4 in CD3/CD28-activated T cells. T cells were stimulated for 48 h with anti-CD3/CD28 and hIL-2. At 48 h of incubation, 0.1 µM GA or DMSO was added, and the cells were incubated for an additional 24 h. The graph shows the mean ± SEM. ns, not significant. DMSO, dimethyl sulfoxide; GA, geldanamycin.
