Table 1.
LC50 values for the pyrethroids bifenthrin and etofenprox, tested on several T. urticae strains, with resistance ratios (RR) calculated relative to IT3 and RO1 in the red and green strains, respectively, and the percentage of reads that align with the voltage-gated sodium channel (VGSC, tetur35g00970) and harbor resistance mutations. (n = number of mites, LC50 = lethal concentration for 50% of the subjects, df = degrees of freedom, SE = standard error, CI = confidence interval, RR = resistance ratio, df = degrees of freedom.)
| Bifenthrin | Etofenprox | VGSC Mutations | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Strain | n | χ² (df) |
Slope (±SE) |
LC50 (95% CI) |
RR (95%CI) |
n | χ² (df) |
Slope (±SE) |
LC50 (95% CI) |
RR (95% CI) |
M918L | L925M | L1024V | F1534S | F1538I |
| El Juan | 490 | 43.27 (18) |
1.82 (±0.19) |
2.9 (2.0–3.9) |
2.2 a (1.4–3.4) |
585 | 54.58 (18) |
3.13 (±0.46) |
24 (17–30) |
1.02 a (0.84–1.2) |
- | - | - | - | - |
| SR6i | 1120 | 81.58 (38) |
1.76 (±0.12) |
5.4 (4.4–6.4) |
4.0 a (2.6–6.2) |
520 | 8.74 (18) |
2.61 (±0.24) |
6.9 (5.9–7.9) |
0.30 a (0.24–0.37) |
- | - | - | - | - |
| MR-VL | 506 | 18.65 (18) |
2.08 (±0.31) |
3400 (2700–4600) |
2500 a (1500–4100) |
479 | 55.26 (18) |
3.36 (±0.34) |
220 (170–270) |
9.4 a (7.8–11) |
- | - | 100% | - | - |
| MR-VLi | 727 | 53.83 (26) |
1.59 (±0.17) |
2400 (1800–3600) |
1800 a (1100–2900) |
445 | 26.83 (18) |
2.19 (±0.21) |
60 (47 -74) |
2.6 a (2.1–3.2) |
- | - | 100% | - | - |
| ES1 | 1082 | 59.18 (38) |
2.06 (±0.48) |
6300 (4200–22,000) |
9500 a (2500–35,000) |
512 | 26.79 (18) |
4.00 (±0.71) |
190 (120–240) |
8.3 a (6.4–11) |
- | - | 33% | - | 25% |
| IT3 | 446 | 24.77 (18) |
0.94 (±0.16) |
1.35 (0.62–2.1) |
/ | 479 | 14.02 (18) |
2.60 (±0.23) |
23 (20–26) |
/ | - | - | - | - | - |
| ROS-IT | 950 | 28.09 (38) |
0.678 (±0.081) |
3500 (2100–7200) |
1470 b (770–2800) |
542 | 8.31 (18) |
2.59 (±0.71) |
93 (20–150) |
9.0 b (4.2–19) |
- | 25% | - | - | - |
| ROS-ITi | 482 | 26.74 (21) |
2.02 (±0.22) |
3.2 (2.4–4.1) |
1.4 b (1.0–1.8) |
536 | 47.89 (21) |
3.03 (±0.36) |
12.5 (9.3–15) |
1.2 b (1.0–1.5) |
- | - | - | - | - |
| UK4 | 1056 | 70.27 (38) |
1.397 (±0.099) |
5.5 (4.0–7.0) |
2.3 b (1.7–3.1) |
458 | 19.98 (18) |
2.99 (±0.27) |
21 (18–24) |
2.0 b (1.7–2.4) |
- | - | - | - | 20% |
| RO1 | 911 | 52.23 (38) |
1.65 (±0.13) |
2.4 (1.7–3.0) |
/ | 426 | 43.21 (18) |
4.23 (±0.45) |
10.3 (8.3–12) |
/ | - | - | - | - | - |
a RR and corresponding 95% CI are calculated relative to the LC50 of IT3; b RR and corresponding 95% CI are calculated relative to the LC50 of RO1. Red strains are underlined.