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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
(A,B) Immunofluorescence staining for 3D SCAP spheres and 2D SCAPs for detecting Nestin and NeuN expression at different time points; (C) Neural markers expression analysis of 2D and 3D SCAPs in two time points, n = 12/group (**: p < 0.001).