Figure 1.

Summary of Amniotic Fluid Collection and Processing. Amniotic fluid is collected during an elective Cesarean delivery prior to removal of the fetus. (A) The top panel denotes the constituents in whole amniotic fluid prior to processing. (B) The bottom panel (below dashed line) describes the preparatory stages of purifying whole amniotic fluid into cell-free amniotic fluid. Upon manual processing including centrifugation and subsequent filtration, the collected eluent is considered cell-free and comprised of vital soluble biomolecules and small insoluble EVs. (Image created with Bio Render).