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. 2022 Nov 9;10(11):2861. doi: 10.3390/biomedicines10112861

Figure 1.

Figure 1

Development of a potency assay for END55. (A) Secreted CTGF protein from human fetal fibroblasts (MRC5) was measured by ELISA following 24 h culture with PBS (vehicle), TGFβ (5 ng/mL), or TGFβ+END55 (200 µg/mL). N = 3. (B) MMP1 protein measured in MRC5 supernatants after incubation with PBS (vehicle), TGFβ (5 ng/mL), or TGFβ+END55 (200 µg/mL) for 24 h. N = 3. (C) Relative gene expression of MMP1 (normalized to B2M) in fibroblasts treated with PBS (vehicle), TGFβ (5 ng/mL), or TGFβ+END55 (100 µg/mL) for 1 h. N = 4. (D) Mouse LOX levels measured in 21-day serum by ELISA. N = 5–10. Data are represented as mean ± SD. * p < 0.05; ** p < 0.01. Bleo, bleomycin.