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. 2022 Nov 3;10(11):2794. doi: 10.3390/biomedicines10112794

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Comparison of the ICOS⁺ and ICOS⁻ Treg levels in the HPV⁺ and HPV⁻ tumors. The numbers of Treg cells with or without ICOS production per Mpx were compared in (A) the parenchyma and (B) the stroma of the tumors of different etiology. Higher ICOS⁺ Treg levels were observed in both the parenchyma and stroma of the HPV⁻ tumors, with the difference being significant for the stroma. Higher ICOS⁻ Treg numbers were detected again in both the parenchyma and stroma of HPV⁺ tumors, with the difference being significant for the parenchyma. * p ≤ 0.05, ** p ≤ 0.01, Mann–Whitney U test.