TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristic(s) | Reference or source |
|---|---|---|
| Y. pestis | ||
| KIM | BG mediavalis | R. R. Brubaker |
| KIM pgm | Spontaneous nonpigmented mutant of KIM | This study |
| Y. pseudotuberculosis | ||
| PB1 | Serotype O:1A | R. R. Brubaker |
| PB1 Nalr | Nalr derivative of PB1 | This study |
| Y. enterocolitica | ||
| WA-314 | Clinical isolate, O:8 BG 1B | 23 |
| WA-C | Plasmidless derivative of WA-314, Nalr | 23 |
| 8081 | Clinical isolate, O:8 BG 1B | 32 |
| Ye1209-79 | Clinical isolate, O:13 BG 1B | 23 |
| Ye1223-75-1 | Clinical isolate, O:20 BG 1B | 23 |
| Ye737 | Clinical isolate, O:21 BG 1B | 23 |
| Y-108 | Clinical isolate, O:3 BG 4 | 23 |
| 108-C | Plasmidless derivative of Y-108 | 23 |
| H567/90 | Clinical isolate, O:5,27 BG 3 | 23 |
| Y-96 | Clinical isolate, O:9 BG 2 | 23 |
| 96-C | Plasmidless derivative of Y-96 | 23 |
| NF-O | Clinical isolate, O:5 BG 1A | 37 |
| E. coli | ||
| Phi | Pesticin sensitive, carrying HPI | R. R. Brubaker |
| K49 | Pesticin sensitive, carrying HPI, ColK, O:156 | 36 |
| K235 | Pesticin sensitive, carrying HPI, ColK, O:1 | 36 |
| D-1041-86 | Enteroaggregative, O:44 | 40 |
| C-4441 | Enteroaggregative, O:128 | 40 |
| 12860 | Enteroinvasive, O:124 | 40 |
| Plasmids | ||
| 12H2 | pLAFR2 cosmid carrying Y. enterocolitica WA-C HPI sequences upstream of the irp2 gene, Tcr | 35 |
| D11 | pLAFR2 cosmid carrying Y. pseudotuberculosis PB1 HPI sequences downstream of the irp1 gene | 36 |
| pRS3 | EcoRI-BamHI fragment of 8081 HPI carrying RS3 | 8 |
| pEBa | BamHI-EcoRI fragment of 8081 between pRS3 and EBg2.4 | 8 |
| EBg2.4 | EcoRI-BglII fragment of 8081 HPI carrying IS1400 | 8 |
| pMOS | Cloning vector, Apr | Amersham |
| pBluescript KSII | Cloning vector, Apr | Stratagene |