TABLE 2.

Primers used in this study

Primer	Primer sequence (5′ to 3′)	Orientation
Integrase gene
int520	ACATCCTTGCGAATCCTTATC	Forward
c15-205	TACAGGCAGGTTCCCGATGAC	Reverse
int1597	CCTGTGGAGGTGGTGGTAAT	Reverse
ybtA promoter
ybt7524	AAACAGGGTCGGGAGAGGATT	Forward
D7	GGACATCGATTCAGTATTGGA	Forward
ybt1012	GCCATAGACGCTGTTGTTGAA	Reverse
D340	GGATTGCGTTTGCGGTGACTC	Reverse
3′ end of Yen HPI
HPI878	GGGGGCAAGAAAAACTAACC	Forward
HPI387	TCATTAAATAGTCACCCCATAG	Forward
Ye9765	CAAGTGTCACCAGCGTAGGGATTTC	Forward
HPI1220	TTTGTTTTATGGCTTTGGTAG	Reverse
HPI283	TTTCAGTGTTTCAGCGTCCAG	Reverse
3P6057	CAACTTGAGCGTGATAAACA	Reverse
Left junction of Yen HPI
W250	TCGGCTCGACGCTTTAGGTAG	Forward
W598	CCAATTTTTCCCGATGCGTAG	Reverse
Right junction of HPI
3P345	GAGGCGATCCCAGTCAGAG	Forward
Ye262	TTTTCCCCCGAGAGGCTGAGTAACC	Reverse for Yen HPI
W36878	TGGCTTCCCCGCTTTTTATCGTAT	Reverse for Yps HPI
asn tRNA gene
asn468	CCGTATGTCACTGGTTCG	Forward