Figure 1.

Dopamine neuron numbers and tyrosine hydroxylase levels are not altered by VPA prenatal exposure. (A) No significant difference is observed in TH+ cell number within the ventral mesencephalon in response to VPA prenatal exposure. Saline males (n = 12); VPA males (n = 7). On the left of panel A is a representative picture showing the area (black rectangle) where TH positive neurons were quantified. (B) No alterations were found in striatal and accumbal TH levels. Striatum: saline males (n = 6), VPA males (n = 6) and nucleus accumbens: saline males (n = 5), VPA males (n = 5). On the left of panel B is a representative immunoblot showing TH (top) and tubulin (bottom) bands. All data are expressed as means ± SEM; Student’s t-tests were performed.