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Validation of Aβ42 fibril induced astrocyte genes by immunolabeling. Increased immunolabeling for iNOS, GBP2, and C3D co-localized with astrocytes surrounding amyloid plaques. Brain sections from 15 M WT rats (A,C,E) and F344Tg-AD rats (B,D,F) were labeled with mAb66.1 to detect amyloid plaques (blue) and goat polyclonal antibody to GFAP for astrocytes (red), as well as rabbit polyclonal antibodies to iNOS, GBP2, and C3D (green). Scale bars = 50 µm.
