Figure 1.

Schematic overview of the PDGFB-driven glioma mouse model using the RCAS-tva delivery system. (A) Schematic overview of the RCAS plasmid including a PDGFB amplification. This vector is transfected into DF-1 cells. (B) Viral replication takes place after cellular entry via tva receptor binding. (C) Implantation of 5 × 10⁴ transfected DF-1 cells into genetic engineered animals. Tissue-dependent expression of the tva receptor is ensured by expression of tva controlled by the nestin promoter in mice. This leads to the infection of only the nestin-positive cell population (red). Subsequently, the PDGFB amplification is integrated retrogradely into the host genome. Together with the systemic deletion of the cell cycle regulator Cdkn2a, intracerebral tumor formation occurs. Created with BioRender.com.