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2-D Western blots of isolated CSL (6 μg) demonstrating antigen species migrating in the 1,200- to 1,400-kDa region (arrows) which are recognized by MAb 3E2 (A) or MAb 4D10 (B). Note the recognition of species 1 and 2 by 3E2 but not by 4D10. Molecular mass standards (titin, 2,450 kDa; nebulin, 770 kDa) and pI values are indicated on the left and bottom, respectively, of each panel.
