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. 2022 Nov 21;11(22):3692. doi: 10.3390/cells11223692

Figure 1.

Figure 1

Preparation of CAR-Ms and their CAR expression. (A) Schematic representation of lentiviral vectors expressing CAR or GFP control. SP, signal peptide; TM, transmembrane domain. (B) Schematic of CAR-M production using lentivirus. (C) Flow cytometric analysis of GFP and CAR expression in bone marrow derived macrophages (BMDM). Data are representative of three independent experiments. (D) Flow cytometric analysis of macrophage polarization M1 and M2 surface markers (CD86, CD80, CD206). UTD-M, untransduced macrophage. Data are representative of three independent experiments.