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. 2022 Oct 25;12(11):1436. doi: 10.3390/brainsci12111436

Figure 7.

Figure 7

GABAARα1 negatively regulates TLR4 expression. Using TAK-242 to inhibit TLR4 or Muscimol to activate GABAARα1, Protein was collected from the temporal cortex (A), hippocampus (D), medullary dorsal horn (G), and trigeminal ganglion (J) of comorbidity, comorbidity + TAK-242, and comorbidity + Muscimol rats. Protein extracts were immunoblotted with antibodies to TLR4, GABAARα1, GAPDH used as a gel loading control. The results are expressed as densitometric units normalized to GAPDH ± SEM. The TLR4 levels were lower in comorbidity + TAK-242 and comorbidity + Muscimol groups compared with the comorbidity group (n = 3, * p < 0.05, ** p < 0.01) (B,E,H,K). The GABAARα1 levels were higher in comorbidity + Muscimol group compared with the comorbidity and comorbidity + TAK-242 groups (n = 3, * p < 0.05, ** p < 0.01) (C,F,I,L). TC: temporal cortex, H: hippocampus, DH: medullary dorsal horn, TG: trigeminal ganglion, C: comorbidity.