Figure 2.

Designer PPR proteins of the PPR2.0 scaffold have higher binding activities and selectivities than those of the SCD scaffold. (a) RBP-ELISA for SCD and PPR2.0 scaffold proteins. Four PPR proteins were subjected to analysis using their respective target sequences (t1, t2, t3, and t4) or two non-target sequences (N1 and N2). (b) RNA binding selectivity. The selectivities of the SCD and PPR2.0 scaffolds were examined and plotted by estimating relative intensities, which are the signal intensities of target RNA against those of non-target RNA.