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Immunohistochemical staining of immune-related molecules. (A) CTLA-4+ cells were detected on the stroma and tumor. (B) The expression of CD86 was detected on the lymphocytes. (C–H) CD8 and CD4 positive cells were divided into tCD8+/tCD4+ cells (lymphocytes on the tumors, (C,D)), sCD8+/sCD4+ cells (lymphocytes on the stroma where the curve labeled, (E,F)), and iCD8+/iCD4+ cells (lymphocytes on the invasive front, (G,H)).
