FIG. 2.

Growth curves of CP9 (wild type) and CPI-2 (ure1) in human urine, incubated at 37°C and shaken at 90 rpm. Cultures were grown overnight at 37°C in LB medium with or without kanamycin (40 μg/ml) and diluted in urine to achieve an A₆₀₀ of approximately 0.03. Aliquots were removed over time, and growth was measured by A₆₀₀. 1a, CP9 in urine 1; 1b, CPI-2 in urine 1; 2a, CP9 in urine 2; 2b, CPI-2 in urine 2.