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. 2022 Nov 8;14(22):5487. doi: 10.3390/cancers14225487

Figure 5.

Figure 5

Metabolic activity of T24 cells after treatment with DOX in the absence and presence of collagen. (a) Visualization of mitochondrial membrane potential using fluorescence microscopy and TMRM indicator. Scale bar is 50 µm, applicable to all images. Fluorescence intensity of TMRM in cells. Mean ± SD. * Statistically significant difference from the control value. # Statistically significant difference from T24 cells without collagen at the selected point. (b) Analysis of the expression level of metabolic genes HK1, HK2, LDHA, G6PD, PDK1, PDP1, OGDH using RT-PCR. Expression was normalized to the values for the T24 cells. Concentration of DOX for the cell treatment was 50 µg/mL (IC50).