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. 2022 Nov 12;11(22):3583. doi: 10.3390/cells11223583

Figure 4.

Figure 4

Pioglitazone modifies the expression of MITF and SASPs in vitiligo melanocytes. (A) Semi-quantitative real-time PCR was used to measure MITF mRNA expression in NHM and VHM. VHM showed a higher MITF expression level indicating a high grade of differentiation, which was reverted by PGZ treatment at mRNA (B), but not at protein level (C). (D) Representative Western blot of pErk. β-Actin was used as a loading control. (E) PGZ modulated the expression of senescence-associated markers, such as IGFBP3, both mRNA and protein levels, and IGFBP7 and p16 at mRNA level. (F) In vitiligo melanocytes, PGZ induced a reduction in IL-6 expression quantified by ELISA assay. (G) The relative Cox2 mRNA expression was evaluated after 6 h of treatment with PGZ (2 μM) and measured by qRT-PCR upon normalization to a reference gene (β-Actin), * p ≤ 0.05 and ** p ≤ 0.01.