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. 2022 Nov 17;11(22):3640. doi: 10.3390/cells11223640

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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RT-PCR analysis in iPSC-derived RPE cells. Different regions of RPE65 were amplified in non-treated RPE cells (NT) and RPE cells upon treating the cells with the normal dose (+CHX 1×) or double dose (+CHX 2×) of cycloheximide for both the control (CON) and patient (PAT RPE cells. All amplicons showed RPE65 reduction in PAT RPE. * indicates primer dimers. BEST1 was used as RPE marker detection and ACTB was used as a loading control. MQ: milliQ water; EL: exon-elongation; correct: wild-type transcript; bp: base-pairs.